Acid Casein Peptone

For the cultivation of heterofermentative lactic acid bacteria including lactobacilli, Leuconostoc and lactic acid streptococciApplicationsCultivationLACTIC ACID BACTERIAPackagingSuspend 46 grams of the medium in one litre of destilled water. Add 0.1 grams of cicloheximide (dissolved in a minimum amount of 40% ethanol) and 0.2 ml of Tween 80. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°Cfor 15 minutes.FormulaPeptone 12.5Yeast Extract 7.5D(+)Glucose 10Sodium Citrate 5Thiamine Hydrochloride 0.001Sodium Chloride 5Dipotassium Hydrogen phosphate 5Manganese Chloride 0.14Ferrous sulfate 0.04Magnesium sulfate 0.8